ATTO 594
ATTO 594 (Ex-Max 601 nm/Em-Max 627 nm)
ATTO 594 from ATTO-TEC has an excitation peak at 601 nm and an emission peak at 627 nm. ATTO 594 is a novel fluorescent label belonging to the class of rhodamine dyes. Characteristic features of the label are strong absorption, high fluorescence quantum yield, high thermal and photo-stability, and excellent water solubility. Thus ATTO 594 is highly suitable for single-molecule detection applications and high-resolution microscopy such as PALM, dSTORM, STED etc. Additionally the dye highly qualifies to be applied in flow cytometry (FACS), fluorescence in-situ hybridization (FISH) and many more. ATTO 594 is very hydrophilic and shows a very good water solubility. After coupling to a substrate the label carries a net electrical charge of -1.
Excitation & emission spectra
Key facts
- Excitation maximum
- 601 nm
- Emission maximum
- 627 nm
- Optimal laser line
- 594
- Optimal filter
- 610/20
- Relative brightness
- 2 of 5
- Extinction coefficient
- 120000
- Quantum yield
- 0.85
- Fluorescence lifetime
- 3.9 ns
- Molecular weight
- 1261
- Commercial antibodies
- 1.6k from 9 suppliers
ATTO 594 has an excitation maximum at 601 nm and an emission maximum at 627 nm, a Stokes shift of 26 nm. Its extinction coefficient is 120000 M⁻¹cm⁻¹. It is optimally excited by the 594 laser line.
Frequently asked questions
- What is ATTO 594?
- ATTO 594 from ATTO-TEC has an excitation peak at 601 nm and an emission peak at 627 nm. ATTO 594 is a novel fluorescent label belonging to the class of rhodamine dyes. Characteristic features of the label are strong absorption, high fluorescence quantum yield, high thermal and photo-stability, and excellent water solubility. Thus ATTO 594 is highly suitable for single-molecule detection applications and high-resolution microscopy such as PALM, dSTORM, STED etc. Additionally the dye highly qualifies to be applied in flow cytometry (FACS), fluorescence in-situ hybridization (FISH) and many more. ATTO 594 is very hydrophilic and shows a very good water solubility. After coupling to a substrate the label carries a net electrical charge of -1.
- What laser excites ATTO 594?
- ATTO 594 is optimally excited by the 594 laser line. Its excitation maximum is 601 nm.
- What filter should I use to detect ATTO 594?
- A 610/20 filter captures the peak of the ATTO 594 emission near 627 nm with minimal spillover.
- How bright is ATTO 594?
- ATTO 594 has a relative brightness of 2 out of 5 (quantum yield 0.85).
- How many antibodies are available conjugated to ATTO 594?
- FluoroFinder tracks 1588 commercially available ATTO 594 conjugates, searchable by target, clone, host and application.