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JC-1
JC-1 (Ex-Max 499 nm/Em-Max 594 nm)
JC-1 is a ratiometric probe used in cell health and apoptosis applications to detect changes to the polarity of the mitochondrial membrane. As the mitochondrial membrane potential neutralizes, this dye shifts to a monomeric form with an emission peak at 520 nm (green). When the mitochondrial potential is healthy, the probe aggregates in the membrane causing a shift it its emission to 590 nm (orange).
Excitation & emission spectra
Key facts
- Excitation maximum
- 499 nm
- Emission maximum
- 594 nm
- Optimal laser line
- 488
- Optimal filter
- 585/42
JC-1 has an excitation maximum at 499 nm and an emission maximum at 594 nm, a Stokes shift of 95 nm. It is optimally excited by the 488 laser line.
Frequently asked questions
- What is JC-1?
- JC-1 is a ratiometric probe used in cell health and apoptosis applications to detect changes to the polarity of the mitochondrial membrane. As the mitochondrial membrane potential neutralizes, this dye shifts to a monomeric form with an emission peak at 520 nm (green). When the mitochondrial potential is healthy, the probe aggregates in the membrane causing a shift it its emission to 590 nm (orange).
- What laser excites JC-1?
- JC-1 is optimally excited by the 488 laser line. Its excitation maximum is 499 nm.
- What filter should I use to detect JC-1?
- A 585/42 filter captures the peak of the JC-1 emission near 594 nm with minimal spillover.