APC-CF750
APC-CF®750 (Ex-Max 651 nm/Em-Max 777 nm)
APC-CF®750 from Biotium has an excitation peak at 651 nm and an emission peak at 777 nm and is most commonly used in flow cytometry. Spectrally its similar to APC-Cy7, APC-Alexa 750, APC-Fire™ 750 and APC-H7
Excitation & emission spectra
Key facts
- Excitation maximum
- 651 nm
- Emission maximum
- 777 nm
- Optimal laser line
- 628-640
- Optimal filter
- 780/60
- Relative brightness
- 2 of 5
- Commercial antibodies
- 10 from 2 suppliers
APC-CF750 has an excitation maximum at 651 nm and an emission maximum at 777 nm, a Stokes shift of 126 nm. It is optimally excited by the 628-640 laser line.
Frequently asked questions
- What is APC-CF750?
- APC-CF®750 from Biotium has an excitation peak at 651 nm and an emission peak at 777 nm and is most commonly used in flow cytometry. Spectrally its similar to APC-Cy7, APC-Alexa 750, APC-Fire™ 750 and APC-H7
- What laser excites APC-CF750?
- APC-CF750 is optimally excited by the 628-640 laser line. Its excitation maximum is 651 nm.
- What filter should I use to detect APC-CF750?
- A 780/60 filter captures the peak of the APC-CF750 emission near 777 nm with minimal spillover.
- How bright is APC-CF750?
- APC-CF750 has a relative brightness of 2 out of 5.
- How many antibodies are available conjugated to APC-CF750?
- FluoroFinder tracks 10 commercially available APC-CF750 conjugates, searchable by target, clone, host and application.