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CellTracker Blue

CellTracker Blue (Ex-Max 353 nm/Em-Max 466 nm)

CellTracker® Blue CMAC (7-amino-4-chloromethylcoumarin) is a fluorescent dye used for cell localization and tracking. This probe has an excitation peak at 353 nm and an emission peak at 466 nm.

Excitation & emission spectra

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Key facts

Excitation maximum
353 nm
Emission maximum
466 nm
Optimal laser line
355
Optimal filter
450/50
Commercial antibodies
3 from 1 supplier

CellTracker Blue has an excitation maximum at 353 nm and an emission maximum at 466 nm, a Stokes shift of 113 nm. It is optimally excited by the 355 laser line.

Frequently asked questions

What is CellTracker Blue?
CellTracker® Blue CMAC (7-amino-4-chloromethylcoumarin) is a fluorescent dye used for cell localization and tracking. This probe has an excitation peak at 353 nm and an emission peak at 466 nm.
What laser excites CellTracker Blue?
CellTracker Blue is optimally excited by the 355 laser line. Its excitation maximum is 353 nm.
What filter should I use to detect CellTracker Blue?
A 450/50 filter captures the peak of the CellTracker Blue emission near 466 nm with minimal spillover.
How many antibodies are available conjugated to CellTracker Blue?
FluoroFinder tracks 3 commercially available CellTracker Blue conjugates, searchable by target, clone, host and application.