eBFP
eBFP (Ex-Max 380 nm/Em-Max 440 nm)
eBFP is a dimeric fluorescent protein derived from Aequorea victoria. It has an excitation peak at 380 nm and an emission peak at 440 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 380 nm
- Emission maximum
- 440 nm
- Optimal laser line
- 405
- Optimal filter
- 450/50
- Extinction coefficient
- 31500
- Quantum yield
- 0.2
- Molecular weight
- 26900
eBFP has an excitation maximum at 380 nm and an emission maximum at 440 nm, a Stokes shift of 60 nm. Its extinction coefficient is 31500 M⁻¹cm⁻¹. It is optimally excited by the 405 laser line.
Frequently asked questions
- What is eBFP?
- eBFP is a dimeric fluorescent protein derived from Aequorea victoria. It has an excitation peak at 380 nm and an emission peak at 440 nm.
- What laser excites eBFP?
- eBFP is optimally excited by the 405 laser line. Its excitation maximum is 380 nm.
- What filter should I use to detect eBFP?
- A 450/50 filter captures the peak of the eBFP emission near 440 nm with minimal spillover.