eCFP
eCFP (Ex-Max 434 nm/Em-Max 477 nm)
eCFP is a monomeric fluorescent protein derived from Aequorea victoria. It has an excitation peak at 434 nm and an emission peak at 477 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 434 nm
- Emission maximum
- 477 nm
- Optimal laser line
- 405
- Optimal filter
- 450/50
- Extinction coefficient
- 32500
- Quantum yield
- 0.4
- Molecular weight
- 26900
eCFP has an excitation maximum at 434 nm and an emission maximum at 477 nm, a Stokes shift of 43 nm. Its extinction coefficient is 32500 M⁻¹cm⁻¹. It is optimally excited by the 405 laser line.
Frequently asked questions
- What is eCFP?
- eCFP is a monomeric fluorescent protein derived from Aequorea victoria. It has an excitation peak at 434 nm and an emission peak at 477 nm.
- What laser excites eCFP?
- eCFP is optimally excited by the 405 laser line. Its excitation maximum is 434 nm.
- What filter should I use to detect eCFP?
- A 450/50 filter captures the peak of the eCFP emission near 477 nm with minimal spillover.