Nile Red
Nile Red (Ex-Max 516 nm/Em-Max 578 nm)
Nile Red is a chromogenic and fluorescent lipophilic probe that can be used as a histological stain or a fluorescent probe for lipids, membrane bound organelles or cell membrane. Nile Red excites at 520nm and emits at 580 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 520 nm
- Emission maximum
- 580 nm
- Optimal laser line
- 488-561
- Optimal filter
- 585/42
- Commercial antibodies
- 2 from 2 suppliers
Nile Red has an excitation maximum at 520 nm and an emission maximum at 580 nm, a Stokes shift of 60 nm. It is optimally excited by the 488-561 laser line.
Frequently asked questions
- What is Nile Red?
- Nile Red is a chromogenic and fluorescent lipophilic probe that can be used as a histological stain or a fluorescent probe for lipids, membrane bound organelles or cell membrane. Nile Red excites at 520nm and emits at 580 nm.
- What laser excites Nile Red?
- Nile Red is optimally excited by the 488-561 laser line. Its excitation maximum is 520 nm.
- What filter should I use to detect Nile Red?
- A 585/42 filter captures the peak of the Nile Red emission near 580 nm with minimal spillover.
- How many antibodies are available conjugated to Nile Red?
- FluoroFinder tracks 2 commercially available Nile Red conjugates, searchable by target, clone, host and application.