BCECF (Red em) pH5.2
BCECF (Red em) pH5.2 (Ex-Max 482 nm/Em-Max 525 nm)
BCECF is a pH indicator in its free acid form with a pKa (6.97). This form can be used to construct a standard curve or as a control for the cell-permeant version of the dye, BCECF-AM. At low pH, the reagent is weakly fluorescent but increases in intensity with increasing pH. This probe can be used ratiometrically as the excitation spectrum shifts between 439 nm and 505 nm, pH depending. This probe has, therefor, a primary excitation peak at 505 nm and a secondary peak at 439 nm with an emission peak that remains constant at 524 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 482 nm
- Emission maximum
- 525 nm
- Optimal laser line
- 488
- Optimal filter
- 525/50
- Commercial antibodies
- 1 from 1 supplier
BCECF (Red em) pH5.2 has an excitation maximum at 482 nm and an emission maximum at 525 nm, a Stokes shift of 43 nm. It is optimally excited by the 488 laser line.
Frequently asked questions
- What is BCECF (Red em) pH5.2?
- BCECF is a pH indicator in its free acid form with a pKa (6.97). This form can be used to construct a standard curve or as a control for the cell-permeant version of the dye, BCECF-AM. At low pH, the reagent is weakly fluorescent but increases in intensity with increasing pH. This probe can be used ratiometrically as the excitation spectrum shifts between 439 nm and 505 nm, pH depending. This probe has, therefor, a primary excitation peak at 505 nm and a secondary peak at 439 nm with an emission peak that remains constant at 524 nm.
- What laser excites BCECF (Red em) pH5.2?
- BCECF (Red em) pH5.2 is optimally excited by the 488 laser line. Its excitation maximum is 482 nm.
- What filter should I use to detect BCECF (Red em) pH5.2?
- A 525/50 filter captures the peak of the BCECF (Red em) pH5.2 emission near 525 nm with minimal spillover.
- How many antibodies are available conjugated to BCECF (Red em) pH5.2?
- FluoroFinder tracks 1 commercially available BCECF (Red em) pH5.2 conjugates, searchable by target, clone, host and application.