MitoTracker Red
MitoTracker Red (Ex-Max 575 nm/Em-Max 595 nm)
MitoTracker Red CMXRos from Thermo Fisher Scientific is a potential-dependent mitochondrial localization probe with an excitation peak at 575 nm and an emission peak at 595 nm. It is recommended for fluorescence microscopy and live-cell imaging. It is retained with fixation using paraformaldehyde. This probe is also available in a reduced version that can aid in reducing background signal. The reduced version, MitoTracker Red CM-H2Ros, becomes more intensely fluorescent when it is oxidzed at the mitochondrial membrane.
Excitation & emission spectra
Key facts
- Excitation maximum
- 575 nm
- Emission maximum
- 595 nm
- Optimal laser line
- 561
- Optimal filter
- 585/42
- Commercial antibodies
- 2 from 1 supplier
MitoTracker Red has an excitation maximum at 575 nm and an emission maximum at 595 nm, a Stokes shift of 20 nm. It is optimally excited by the 561 laser line.
Frequently asked questions
- What is MitoTracker Red?
- MitoTracker Red CMXRos from Thermo Fisher Scientific is a potential-dependent mitochondrial localization probe with an excitation peak at 575 nm and an emission peak at 595 nm. It is recommended for fluorescence microscopy and live-cell imaging. It is retained with fixation using paraformaldehyde. This probe is also available in a reduced version that can aid in reducing background signal. The reduced version, MitoTracker Red CM-H2Ros, becomes more intensely fluorescent when it is oxidzed at the mitochondrial membrane.
- What laser excites MitoTracker Red?
- MitoTracker Red is optimally excited by the 561 laser line. Its excitation maximum is 575 nm.
- What filter should I use to detect MitoTracker Red?
- A 585/42 filter captures the peak of the MitoTracker Red emission near 595 nm with minimal spillover.
- How many antibodies are available conjugated to MitoTracker Red?
- FluoroFinder tracks 2 commercially available MitoTracker Red conjugates, searchable by target, clone, host and application.