Cerulean
Cerulean (Ex-Max 433 nm/Em-Max 475 nm)
Cerulean (mCFP) is a blue-emitting monomeric fluorescent protein with an excitation peak at 433 nm and an emission peak at 475 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 433 nm
- Emission maximum
- 475 nm
- Optimal laser line
- 405
- Optimal filter
- 450/50
- Extinction coefficient
- 43000
- Quantum yield
- 0.62
- Molecular weight
- 26800
Cerulean has an excitation maximum at 433 nm and an emission maximum at 475 nm, a Stokes shift of 42 nm. Its extinction coefficient is 43000 M⁻¹cm⁻¹. It is optimally excited by the 405 laser line.
Frequently asked questions
- What is Cerulean?
- Cerulean (mCFP) is a blue-emitting monomeric fluorescent protein with an excitation peak at 433 nm and an emission peak at 475 nm.
- What laser excites Cerulean?
- Cerulean is optimally excited by the 405 laser line. Its excitation maximum is 433 nm.
- What filter should I use to detect Cerulean?
- A 450/50 filter captures the peak of the Cerulean emission near 475 nm with minimal spillover.