DiO
DiO (Ex-Max 487 nm/Em-Max 504 nm)
DiO is a lipophilic carbocyanine dye often used in fluorescence microscopy to label the cell surface, trace neuronal axons or, upon fixation, any lipid bound compartment. This probe has an excitation peak at 487 nm and an emission peak at 504 nm
Excitation & emission spectra
Key facts
- Excitation maximum
- 487 nm
- Emission maximum
- 504 nm
- Optimal laser line
- 488
- Optimal filter
- 515/30
- Molecular weight
- 881.7173
- Commercial antibodies
- 6 from 2 suppliers
DiO has an excitation maximum at 487 nm and an emission maximum at 504 nm, a Stokes shift of 17 nm. It is optimally excited by the 488 laser line.
Frequently asked questions
- What is DiO?
- DiO is a lipophilic carbocyanine dye often used in fluorescence microscopy to label the cell surface, trace neuronal axons or, upon fixation, any lipid bound compartment. This probe has an excitation peak at 487 nm and an emission peak at 504 nm
- What laser excites DiO?
- DiO is optimally excited by the 488 laser line. Its excitation maximum is 487 nm.
- What filter should I use to detect DiO?
- A 515/30 filter captures the peak of the DiO emission near 504 nm with minimal spillover.
- How many antibodies are available conjugated to DiO?
- FluoroFinder tracks 6 commercially available DiO conjugates, searchable by target, clone, host and application.