PE-Dazzle 594
PE-Dazzle 594 (Ex-Max 565 nm/Em-Max 610 nm)
PE-Dazzle® 594 (RPE-Dazzle 594) from Biolegend is a red-emitting tandem fluorophore that combines pycoerythrin (PE) and Dazzle 594. The donor molecule, PE can be excited by the 488-nm blue, 532-nm green, or 561-nm yellow-green laser and and transfers energy to the acceptor molecule, Dazzle 594, which emitts light that can be captured with a 610/20 nm bandpass filter. PE-Dazzle 594 has an excitation peak at 565 nm and an emission peak at 610 nm, and is suitable alternative to PE-Texas Red, PE-eF610, PE-Alexa Fluor™ 610 or PE-CF®594.
Excitation & emission spectra
Key facts
- Excitation maximum
- 565 nm
- Emission maximum
- 610 nm
- Optimal laser line
- 488-561
- Optimal filter
- 610/20
- Relative brightness
- 5 of 5
- Molecular weight
- 488000
- Commercial antibodies
- 1.9k from 3 suppliers
PE-Dazzle 594 has an excitation maximum at 565 nm and an emission maximum at 610 nm, a Stokes shift of 45 nm. It is optimally excited by the 488-561 laser line.
Frequently asked questions
- What is PE-Dazzle 594?
- PE-Dazzle® 594 (RPE-Dazzle 594) from Biolegend is a red-emitting tandem fluorophore that combines pycoerythrin (PE) and Dazzle 594. The donor molecule, PE can be excited by the 488-nm blue, 532-nm green, or 561-nm yellow-green laser and and transfers energy to the acceptor molecule, Dazzle 594, which emitts light that can be captured with a 610/20 nm bandpass filter. PE-Dazzle 594 has an excitation peak at 565 nm and an emission peak at 610 nm, and is suitable alternative to PE-Texas Red, PE-eF610, PE-Alexa Fluor™ 610 or PE-CF®594.
- What laser excites PE-Dazzle 594?
- PE-Dazzle 594 is optimally excited by the 488-561 laser line. Its excitation maximum is 565 nm.
- What filter should I use to detect PE-Dazzle 594?
- A 610/20 filter captures the peak of the PE-Dazzle 594 emission near 610 nm with minimal spillover.
- How bright is PE-Dazzle 594?
- PE-Dazzle 594 has a relative brightness of 5 out of 5.
- How many antibodies are available conjugated to PE-Dazzle 594?
- FluoroFinder tracks 1857 commercially available PE-Dazzle 594 conjugates, searchable by target, clone, host and application.