Brilliant Violet 786
Brilliant™ Violet 786 (Ex-Max 405 nm/Em-Max 785 nm)
Brilliant™ Violet 786 (BV786) is a far-red-emitting tandem fluorophore that can be excited by the 405 nm Violet laser and collected using a 780/60 bandpass filter. BV750 has an excitation peak at 405 nm and an emission peak at 786 nm. Due to its unique Stokes shift, few dyes have identical or near identical excitation and emission spectrums, but the nearest alternatives are Qdot® 800 and SuperBright 780 (ThermoFisher), and Brilliant™ Violet 785 (BioLegend). What's the difference between BV785 and BV786? BV785 is a BioLegend color, while BV786 is a BD Biosciences color, but they exhibit the same spectral properties. This dye exhibits a medium level of brightness and is most often used in flow cytometry. Older instruments may not be set up for a dye with such a large Stokes shift, however BV786 is especially well suited for spectral cytometers or sorters. This dye is part of the Brilliant™ Violet dye line of fluorescent polymers. Brilliant™ Violet 421 polymer is employed as the donor molecule in a series of tandem dyes with acceptor molecules emitting at various points across the visible light spectrum. The Brilliant™ Violet dyes are a superior alternative to QDot nanocrystals and similar to SuperNova dye from Beckman Coulter and StarBright dyes from Bio-Rad.
Excitation & emission spectra
Key facts
- Excitation maximum
- 405 nm
- Emission maximum
- 786 nm
- Optimal laser line
- 405
- Optimal filter
- 780/60
- Relative brightness
- 3 of 5
- Extinction coefficient
- 2500000
- Quantum yield
- 0.04
- Molecular weight
- 60000
- Commercial antibodies
- 1.1k from 3 suppliers
Brilliant Violet 786 has an excitation maximum at 405 nm and an emission maximum at 786 nm, a Stokes shift of 381 nm. Its extinction coefficient is 2500000 M⁻¹cm⁻¹. It is optimally excited by the 405 laser line.
Frequently asked questions
- What is Brilliant Violet 786?
- Brilliant™ Violet 786 (BV786) is a far-red-emitting tandem fluorophore that can be excited by the 405 nm Violet laser and collected using a 780/60 bandpass filter. BV750 has an excitation peak at 405 nm and an emission peak at 786 nm. Due to its unique Stokes shift, few dyes have identical or near identical excitation and emission spectrums, but the nearest alternatives are Qdot® 800 and SuperBright 780 (ThermoFisher), and Brilliant™ Violet 785 (BioLegend). What's the difference between BV785 and BV786? BV785 is a BioLegend color, while BV786 is a BD Biosciences color, but they exhibit the same spectral properties. This dye exhibits a medium level of brightness and is most often used in flow cytometry. Older instruments may not be set up for a dye with such a large Stokes shift, however BV786 is especially well suited for spectral cytometers or sorters. This dye is part of the Brilliant™ Violet dye line of fluorescent polymers. Brilliant™ Violet 421 polymer is employed as the donor molecule in a series of tandem dyes with acceptor molecules emitting at various points across the visible light spectrum. The Brilliant™ Violet dyes are a superior alternative to QDot nanocrystals and similar to SuperNova dye from Beckman Coulter and StarBright dyes from Bio-Rad.
- What laser excites Brilliant Violet 786?
- Brilliant Violet 786 is optimally excited by the 405 laser line. Its excitation maximum is 405 nm.
- What filter should I use to detect Brilliant Violet 786?
- A 780/60 filter captures the peak of the Brilliant Violet 786 emission near 786 nm with minimal spillover.
- How bright is Brilliant Violet 786?
- Brilliant Violet 786 has a relative brightness of 3 out of 5 (quantum yield 0.04).
- How many antibodies are available conjugated to Brilliant Violet 786?
- FluoroFinder tracks 1130 commercially available Brilliant Violet 786 conjugates, searchable by target, clone, host and application.