APC-Vio770
APC-Vio®770 (Ex-Max 652 nm/Em-Max 775 nm)
APC-Vio®770 from Miltenyi is a tandem fluorophore with APC as the donor and has an excitation peak at 652 nm and an emission peak at 775 nm, and is spectrally similar to APC-Cy® 7 (GE Healthcare) APC-H7 (BD Biosciences), APC-eFluor™ 780 (ThermoFisher Scientific), APC-Vio®770 (Miltenyi Biotec), and APC-Fire™ 750 (BioLegend). The Vio® dye family are products of Miltenyi Biotec, with many antibody conjugates designed and validated for flow cytometry.
Excitation & emission spectra
Key facts
- Excitation maximum
- 652 nm
- Emission maximum
- 775 nm
- Optimal laser line
- 628-640
- Optimal filter
- 780/60
- Relative brightness
- 2 of 5
- Commercial antibodies
- 1.2k from 2 suppliers
APC-Vio770 has an excitation maximum at 652 nm and an emission maximum at 775 nm, a Stokes shift of 123 nm. It is optimally excited by the 628-640 laser line.
Frequently asked questions
- What is APC-Vio770?
- APC-Vio®770 from Miltenyi is a tandem fluorophore with APC as the donor and has an excitation peak at 652 nm and an emission peak at 775 nm, and is spectrally similar to APC-Cy® 7 (GE Healthcare) APC-H7 (BD Biosciences), APC-eFluor™ 780 (ThermoFisher Scientific), APC-Vio®770 (Miltenyi Biotec), and APC-Fire™ 750 (BioLegend). The Vio® dye family are products of Miltenyi Biotec, with many antibody conjugates designed and validated for flow cytometry.
- What laser excites APC-Vio770?
- APC-Vio770 is optimally excited by the 628-640 laser line. Its excitation maximum is 652 nm.
- What filter should I use to detect APC-Vio770?
- A 780/60 filter captures the peak of the APC-Vio770 emission near 775 nm with minimal spillover.
- How bright is APC-Vio770?
- APC-Vio770 has a relative brightness of 2 out of 5.
- How many antibodies are available conjugated to APC-Vio770?
- FluoroFinder tracks 1167 commercially available APC-Vio770 conjugates, searchable by target, clone, host and application.