PE-Vio770
PE-Vio®770 (Ex-Max 565 nm/Em-Max 775 nm)
PE-Vio® 770 from Miltenyi Biotec is a red-emitting tandem fluorophore that combines pycoerythrin (PE) and Vio®770. The donor molecule, PE can be excited by the 488-nm blue, 532-nm green, or 561-nm yellow-green laser and and transfers energy to the acceptor molecule, Vio®770, which emitts light that can be captured with a 780/60 nm bandpass filter. PE-Vio®770 has an excitation peak at 565 nm and an emission peak at 775 nm and is a common alternative to PE-Cy7 and PE-H7.
Excitation & emission spectra
Key facts
- Excitation maximum
- 565 nm
- Emission maximum
- 775 nm
- Optimal laser line
- 488-561
- Optimal filter
- 780/60
- Relative brightness
- 4 of 5
- Extinction coefficient
- 1960000
- Molecular weight
- 121000
- Commercial antibodies
- 1.8k from 2 suppliers
PE-Vio770 has an excitation maximum at 565 nm and an emission maximum at 775 nm, a Stokes shift of 210 nm. Its extinction coefficient is 1960000 M⁻¹cm⁻¹. It is optimally excited by the 488-561 laser line.
Frequently asked questions
- What is PE-Vio770?
- PE-Vio® 770 from Miltenyi Biotec is a red-emitting tandem fluorophore that combines pycoerythrin (PE) and Vio®770. The donor molecule, PE can be excited by the 488-nm blue, 532-nm green, or 561-nm yellow-green laser and and transfers energy to the acceptor molecule, Vio®770, which emitts light that can be captured with a 780/60 nm bandpass filter. PE-Vio®770 has an excitation peak at 565 nm and an emission peak at 775 nm and is a common alternative to PE-Cy7 and PE-H7.
- What laser excites PE-Vio770?
- PE-Vio770 is optimally excited by the 488-561 laser line. Its excitation maximum is 565 nm.
- What filter should I use to detect PE-Vio770?
- A 780/60 filter captures the peak of the PE-Vio770 emission near 775 nm with minimal spillover.
- How bright is PE-Vio770?
- PE-Vio770 has a relative brightness of 4 out of 5.
- How many antibodies are available conjugated to PE-Vio770?
- FluoroFinder tracks 1756 commercially available PE-Vio770 conjugates, searchable by target, clone, host and application.