Hc Red
Hc Red (Ex-Max 592 nm/Em-Max 614 nm)
Hc Red is a tetrameric red fluroescent protein derived from the sea anemones Heteractis crispa. It has an excitation peak at 592 nm and an emission peak at 645 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 592 nm
- Emission maximum
- 614 nm
- Optimal laser line
- 594
- Optimal filter
- 610/20
- Extinction coefficient
- 20000
- Quantum yield
- 0.015
Hc Red has an excitation maximum at 592 nm and an emission maximum at 614 nm, a Stokes shift of 22 nm. Its extinction coefficient is 20000 M⁻¹cm⁻¹. It is optimally excited by the 594 laser line.
Frequently asked questions
- What is Hc Red?
- Hc Red is a tetrameric red fluroescent protein derived from the sea anemones Heteractis crispa. It has an excitation peak at 592 nm and an emission peak at 645 nm.
- What laser excites Hc Red?
- Hc Red is optimally excited by the 594 laser line. Its excitation maximum is 592 nm.
- What filter should I use to detect Hc Red?
- A 610/20 filter captures the peak of the Hc Red emission near 614 nm with minimal spillover.