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Alexa Fluor 660

Alexa Fluor™ 660 (Ex-Max 663 nm/Em-Max 690 nm)

Alexa Fluor™ 660 (AF660,Alexa 660) has an excitation peak at 663nm and an emission peak at 690nm. It can be used with the following analysis platforms: Flow Cytometry, Spectral Flow Cytometry.

Excitation & emission spectra

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Key facts

Excitation maximum
663 nm
Emission maximum
690 nm
Optimal laser line
628-640
Optimal filter
695/40
Relative brightness
3 of 5
Extinction coefficient
132000
Commercial antibodies
260 from 6 suppliers

Alexa Fluor 660 has an excitation maximum at 663 nm and an emission maximum at 690 nm, a Stokes shift of 27 nm. Its extinction coefficient is 132000 M⁻¹cm⁻¹. It is optimally excited by the 628-640 laser line.

Frequently asked questions

What is Alexa Fluor 660?
Alexa Fluor™ 660 (AF660,Alexa 660) has an excitation peak at 663nm and an emission peak at 690nm. It can be used with the following analysis platforms: Flow Cytometry, Spectral Flow Cytometry.
What laser excites Alexa Fluor 660?
Alexa Fluor 660 is optimally excited by the 628-640 laser line. Its excitation maximum is 663 nm.
What filter should I use to detect Alexa Fluor 660?
A 695/40 filter captures the peak of the Alexa Fluor 660 emission near 690 nm with minimal spillover.
How bright is Alexa Fluor 660?
Alexa Fluor 660 has a relative brightness of 3 out of 5.
How many antibodies are available conjugated to Alexa Fluor 660?
FluoroFinder tracks 260 commercially available Alexa Fluor 660 conjugates, searchable by target, clone, host and application.