Alexa Fluor 594
Alexa Fluor™ 594 (Ex-Max 590 nm/Em-Max 617 nm)
Alexa Fluor™ 594 (AF594, Alexa 594) has an excitation peak at 590 nm and an emission peak at 617 nm, and is spectrally similar to Texas Red (ThermoFisher Scientific), DyLight™ 594 (ThermoFisher Scientific), iFluor® 594 (ATT Bioquest) and iFluor® 610 (ATT Bioquest), CF®594 (Biotium), and ATTO 594 (ATTO-TEC). Alexa 594 is commonly used for flow cytometry,fluorescence microscopy and super-resolution microscopy. It is very bright, photostable, and pH insensitive.
Excitation & emission spectra
Key facts
- Excitation maximum
- 590 nm
- Emission maximum
- 617 nm
- Optimal laser line
- 594
- Optimal filter
- 630/69
- Relative brightness
- 2 of 5
- Extinction coefficient
- 73000
- Quantum yield
- 0.66
- Molecular weight
- 800
- Commercial antibodies
- 30.8k from 18 suppliers
Alexa Fluor 594 has an excitation maximum at 590 nm and an emission maximum at 617 nm, a Stokes shift of 27 nm. Its extinction coefficient is 73000 M⁻¹cm⁻¹. It is optimally excited by the 594 laser line.
Frequently asked questions
- What is Alexa Fluor 594?
- Alexa Fluor™ 594 (AF594, Alexa 594) has an excitation peak at 590 nm and an emission peak at 617 nm, and is spectrally similar to Texas Red (ThermoFisher Scientific), DyLight™ 594 (ThermoFisher Scientific), iFluor® 594 (ATT Bioquest) and iFluor® 610 (ATT Bioquest), CF®594 (Biotium), and ATTO 594 (ATTO-TEC). Alexa 594 is commonly used for flow cytometry,fluorescence microscopy and super-resolution microscopy. It is very bright, photostable, and pH insensitive.
- What laser excites Alexa Fluor 594?
- Alexa Fluor 594 is optimally excited by the 594 laser line. Its excitation maximum is 590 nm.
- What filter should I use to detect Alexa Fluor 594?
- A 630/69 filter captures the peak of the Alexa Fluor 594 emission near 617 nm with minimal spillover.
- How bright is Alexa Fluor 594?
- Alexa Fluor 594 has a relative brightness of 2 out of 5 (quantum yield 0.66).
- How many antibodies are available conjugated to Alexa Fluor 594?
- FluoroFinder tracks 30838 commercially available Alexa Fluor 594 conjugates, searchable by target, clone, host and application.