Alexa Fluor 514
Alexa Fluor™ 514 (Ex-Max 518 nm/Em-Max 540 nm)
Alexa Fluor™ 514 (AF514, Alexa 514) has an excitation peak at 518nm and an emission peak at 540nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 518 nm
- Emission maximum
- 540 nm
- Optimal laser line
- 488
- Optimal filter
- 530/43
- Relative brightness
- 1 of 5
- Commercial antibodies
- 9 from 1 supplier
Alexa Fluor 514 has an excitation maximum at 518 nm and an emission maximum at 540 nm, a Stokes shift of 22 nm. It is optimally excited by the 488 laser line.
Frequently asked questions
- What is Alexa Fluor 514?
- Alexa Fluor™ 514 (AF514, Alexa 514) has an excitation peak at 518nm and an emission peak at 540nm.
- What laser excites Alexa Fluor 514?
- Alexa Fluor 514 is optimally excited by the 488 laser line. Its excitation maximum is 518 nm.
- What filter should I use to detect Alexa Fluor 514?
- A 530/43 filter captures the peak of the Alexa Fluor 514 emission near 540 nm with minimal spillover.
- How bright is Alexa Fluor 514?
- Alexa Fluor 514 has a relative brightness of 1 out of 5.
- How many antibodies are available conjugated to Alexa Fluor 514?
- FluoroFinder tracks 9 commercially available Alexa Fluor 514 conjugates, searchable by target, clone, host and application.