Alexa Fluor 350
Alexa Fluor™ 350 (AF350)
Alexa Fluor™ 350 (AF350, Alexa 350) has an excitation peak at 346 nm and an emission peak at 442 nm, and is spectrally similar in size, brightness and application to AMCA, DyLight™ 350 and CF®350. Alexa 350 is most commonly used in flow cytometery and fluorescence microscopy applications.
Excitation & emission spectra
Key facts
- Excitation maximum
- 346 nm
- Emission maximum
- 442 nm
- Optimal laser line
- 355
- Optimal filter
- 450/50
- Relative brightness
- 1 of 5
- Extinction coefficient
- 19000
- Molecular weight
- 410
- Commercial antibodies
- 30.5k from 10 suppliers
Alexa Fluor 350 has an excitation maximum at 346 nm and an emission maximum at 442 nm, a Stokes shift of 96 nm. Its extinction coefficient is 19000 M⁻¹cm⁻¹. It is optimally excited by the 355 laser line.
Frequently asked questions
- What is Alexa Fluor 350?
- Alexa Fluor™ 350 (AF350, Alexa 350) has an excitation peak at 346 nm and an emission peak at 442 nm, and is spectrally similar in size, brightness and application to AMCA, DyLight™ 350 and CF®350. Alexa 350 is most commonly used in flow cytometery and fluorescence microscopy applications.
- What laser excites Alexa Fluor 350?
- Alexa Fluor 350 is optimally excited by the 355 laser line. Its excitation maximum is 346 nm.
- What filter should I use to detect Alexa Fluor 350?
- A 450/50 filter captures the peak of the Alexa Fluor 350 emission near 442 nm with minimal spillover.
- How bright is Alexa Fluor 350?
- Alexa Fluor 350 has a relative brightness of 1 out of 5.
- How many antibodies are available conjugated to Alexa Fluor 350?
- FluoroFinder tracks 30493 commercially available Alexa Fluor 350 conjugates, searchable by target, clone, host and application.