eFluor 660 Fix Viability
eFluor™ 660 Fix Viability (Ex-Max 640 nm/Em-Max 656 nm)
eFluor™ 660 Fixable Viability from Thermo Fisher Scientific is an amine-reactive dye that can be used to discriminate between live and dead cells in flow cytometry. It is compatible with further cryopreservation, fixation or permeabilization. It has an excitaiton peak at 640 nm and an emission peak at 656 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 640 nm
- Emission maximum
- 656 nm
- Optimal laser line
- 628-640
- Optimal filter
- 660/20
eFluor 660 Fix Viability has an excitation maximum at 640 nm and an emission maximum at 656 nm, a Stokes shift of 16 nm. It is optimally excited by the 628-640 laser line.
Frequently asked questions
- What is eFluor 660 Fix Viability?
- eFluor™ 660 Fixable Viability from Thermo Fisher Scientific is an amine-reactive dye that can be used to discriminate between live and dead cells in flow cytometry. It is compatible with further cryopreservation, fixation or permeabilization. It has an excitaiton peak at 640 nm and an emission peak at 656 nm.
- What laser excites eFluor 660 Fix Viability?
- eFluor 660 Fix Viability is optimally excited by the 628-640 laser line. Its excitation maximum is 640 nm.
- What filter should I use to detect eFluor 660 Fix Viability?
- A 660/20 filter captures the peak of the eFluor 660 Fix Viability emission near 656 nm with minimal spillover.