eFluor 520 Fix Viability
eFluor™ 520 Fix Viability (Ex-Max 488 nm/Em-Max 522 nm)
eFluor™ 520 Fixable Viability from Thermo Fisher Scientific is an amine-reactive dye that can be used to discriminate between live and dead cells in flow cytometry. It is compatible with further cryopreservation, fixation or permeabilization. It has an excitaiton peak at 488 nm and an emission peak at 522 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 488 nm
- Emission maximum
- 522 nm
- Optimal laser line
- 488
- Optimal filter
- 525/50
eFluor 520 Fix Viability has an excitation maximum at 488 nm and an emission maximum at 522 nm, a Stokes shift of 34 nm. It is optimally excited by the 488 laser line.
Frequently asked questions
- What is eFluor 520 Fix Viability?
- eFluor™ 520 Fixable Viability from Thermo Fisher Scientific is an amine-reactive dye that can be used to discriminate between live and dead cells in flow cytometry. It is compatible with further cryopreservation, fixation or permeabilization. It has an excitaiton peak at 488 nm and an emission peak at 522 nm.
- What laser excites eFluor 520 Fix Viability?
- eFluor 520 Fix Viability is optimally excited by the 488 laser line. Its excitation maximum is 488 nm.
- What filter should I use to detect eFluor 520 Fix Viability?
- A 525/50 filter captures the peak of the eFluor 520 Fix Viability emission near 522 nm with minimal spillover.