eFluor 455UV Fix Viability
eFluor™ 455UV Fix Viability (Ex-Max 345 nm/Em-Max 450 nm)
eFluor™ 455UV Fixable Viability is an amine-reactive dye that can be used to discriminate between live and dead cells in flow cytometry. It is compatible with further cryopreservation, fixation or permeabilization. It has an excitaiton peak at 345 nm and an emission peak at 455 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 345 nm
- Emission maximum
- 455 nm
- Optimal laser line
- 355
- Optimal filter
- 450/50
eFluor 455UV Fix Viability has an excitation maximum at 345 nm and an emission maximum at 455 nm, a Stokes shift of 110 nm. It is optimally excited by the 355 laser line.
Frequently asked questions
- What is eFluor 455UV Fix Viability?
- eFluor™ 455UV Fixable Viability is an amine-reactive dye that can be used to discriminate between live and dead cells in flow cytometry. It is compatible with further cryopreservation, fixation or permeabilization. It has an excitaiton peak at 345 nm and an emission peak at 455 nm.
- What laser excites eFluor 455UV Fix Viability?
- eFluor 455UV Fix Viability is optimally excited by the 355 laser line. Its excitation maximum is 345 nm.
- What filter should I use to detect eFluor 455UV Fix Viability?
- A 450/50 filter captures the peak of the eFluor 455UV Fix Viability emission near 455 nm with minimal spillover.