Calcein Blue AM
Calcein Blue AM (Ex-Max 359 nm/Em-Max 446 nm)
Calcein Blue, AM, is a fluorogenic blue fluorescent molecule (ex 359/ em 446) that penetrates the membranes of live cells. Once inside the live cell, Calcein Blue, AM is hydrolyzed by intracellular esterases into the fluorescent Calcein Blue which can then be measured via Fluorescence Microscopy, flow cytometer, or fluorescent microplate reader. Reagents that contain an acetoxymethyl ester (AM) or diacetate (DA) side chain, are useful indicators of cell health and vitality, especially when used together with a probe for cell membrane integrity like an impermeant nucleic acid stain or fixable live/dead probe.
Excitation & emission spectra
Key facts
- Excitation maximum
- 359 nm
- Emission maximum
- 446 nm
- Optimal laser line
- 355
- Optimal filter
- 450/50
- Commercial antibodies
- 2 from 2 suppliers
Calcein Blue AM has an excitation maximum at 359 nm and an emission maximum at 446 nm, a Stokes shift of 87 nm. It is optimally excited by the 355 laser line.
Frequently asked questions
- What is Calcein Blue AM?
- Calcein Blue, AM, is a fluorogenic blue fluorescent molecule (ex 359/ em 446) that penetrates the membranes of live cells. Once inside the live cell, Calcein Blue, AM is hydrolyzed by intracellular esterases into the fluorescent Calcein Blue which can then be measured via Fluorescence Microscopy, flow cytometer, or fluorescent microplate reader. Reagents that contain an acetoxymethyl ester (AM) or diacetate (DA) side chain, are useful indicators of cell health and vitality, especially when used together with a probe for cell membrane integrity like an impermeant nucleic acid stain or fixable live/dead probe.
- What laser excites Calcein Blue AM?
- Calcein Blue AM is optimally excited by the 355 laser line. Its excitation maximum is 359 nm.
- What filter should I use to detect Calcein Blue AM?
- A 450/50 filter captures the peak of the Calcein Blue AM emission near 446 nm with minimal spillover.
- How many antibodies are available conjugated to Calcein Blue AM?
- FluoroFinder tracks 2 commercially available Calcein Blue AM conjugates, searchable by target, clone, host and application.