Calcein AM
Calcein AM (Ex-Max 491 nm/Em-Max 511 nm)
Calcein, AM, also called Calcein Green, AM, is a fluorogenic green fluorescent molecule (ex 491/ em 511) that penetrates the membranes of live cells. Once inside the live cell, Calcein, AM is hydrolyzed by intracellular esterases into the fluorescent Calcein which can then be measured via Fluorescence Microscopy, flow cytometer, or fluorescent microplate reader. Reagents that contain an acetoxymethyl ester (AM) or diacetate (DA) side chain, are useful indicators of cell health and vitality, especially when used together with a probe for cell membrane integrity like an impermeant nucleic acid stain or fixable live/dead probe.
Excitation & emission spectra
Key facts
- Excitation maximum
- 491 nm
- Emission maximum
- 511 nm
- Optimal laser line
- 488
- Optimal filter
- 525/50
- Molecular weight
- 994.87
- Commercial antibodies
- 2 from 2 suppliers
Calcein AM has an excitation maximum at 491 nm and an emission maximum at 511 nm, a Stokes shift of 20 nm. It is optimally excited by the 488 laser line.
Frequently asked questions
- What is Calcein AM?
- Calcein, AM, also called Calcein Green, AM, is a fluorogenic green fluorescent molecule (ex 491/ em 511) that penetrates the membranes of live cells. Once inside the live cell, Calcein, AM is hydrolyzed by intracellular esterases into the fluorescent Calcein which can then be measured via Fluorescence Microscopy, flow cytometer, or fluorescent microplate reader. Reagents that contain an acetoxymethyl ester (AM) or diacetate (DA) side chain, are useful indicators of cell health and vitality, especially when used together with a probe for cell membrane integrity like an impermeant nucleic acid stain or fixable live/dead probe.
- What laser excites Calcein AM?
- Calcein AM is optimally excited by the 488 laser line. Its excitation maximum is 491 nm.
- What filter should I use to detect Calcein AM?
- A 525/50 filter captures the peak of the Calcein AM emission near 511 nm with minimal spillover.
- How many antibodies are available conjugated to Calcein AM?
- FluoroFinder tracks 2 commercially available Calcein AM conjugates, searchable by target, clone, host and application.