PerCP-Cy5.5
PerCP-Cy5.5 (Ex-Max 482 nm/Em-Max 695 nm)
PerCP-Cyanine® 5.5 (PerCP-Cy5.5) is a red-emitting tandem fluorophore that was originally designed to make the PerCP fluorophore more stable and increase signal intensity. The donor molecule, PerCP can be excited by the 488-nm blue laser and and transfers energy to the acceptor molecule, Cy5.5, which emitts light that can be captured with a 695/40 nm bandpass filter. PerCP-Cy5.5 has an excitation peak at 482 nm and an emission peak at 695 nm. There are superior alternatives to PerCP and PerCP-Cy5.5 including RealBlue 705 (BD Biosciences), NovaFluor Blue 690 or PerCP-eFluor™ 710.
Excitation & emission spectra
Key facts
- Excitation maximum
- 482 nm
- Emission maximum
- 695 nm
- Optimal laser line
- 488
- Optimal filter
- 695/40
- Relative brightness
- 2 of 5
- Extinction coefficient
- 350000
- Molecular weight
- 58000
- Commercial antibodies
- 5.5k from 32 suppliers
PerCP-Cy5.5 has an excitation maximum at 482 nm and an emission maximum at 695 nm, a Stokes shift of 213 nm. Its extinction coefficient is 350000 M⁻¹cm⁻¹. It is optimally excited by the 488 laser line.
Frequently asked questions
- What is PerCP-Cy5.5?
- PerCP-Cyanine® 5.5 (PerCP-Cy5.5) is a red-emitting tandem fluorophore that was originally designed to make the PerCP fluorophore more stable and increase signal intensity. The donor molecule, PerCP can be excited by the 488-nm blue laser and and transfers energy to the acceptor molecule, Cy5.5, which emitts light that can be captured with a 695/40 nm bandpass filter. PerCP-Cy5.5 has an excitation peak at 482 nm and an emission peak at 695 nm. There are superior alternatives to PerCP and PerCP-Cy5.5 including RealBlue 705 (BD Biosciences), NovaFluor Blue 690 or PerCP-eFluor™ 710.
- What laser excites PerCP-Cy5.5?
- PerCP-Cy5.5 is optimally excited by the 488 laser line. Its excitation maximum is 482 nm.
- What filter should I use to detect PerCP-Cy5.5?
- A 695/40 filter captures the peak of the PerCP-Cy5.5 emission near 695 nm with minimal spillover.
- How bright is PerCP-Cy5.5?
- PerCP-Cy5.5 has a relative brightness of 2 out of 5.
- How many antibodies are available conjugated to PerCP-Cy5.5?
- FluoroFinder tracks 5472 commercially available PerCP-Cy5.5 conjugates, searchable by target, clone, host and application.