PE-Cy7
PE-Cy7 (Ex-Max 565 nm/Em-Max 778 nm)
PE-Cyanine®7 (PE-Cy7, RPE-Cy7) is a far red-emitting tandem fluorophore that combines phycoerythrin (PE) and Cy7. The donor molecule, PE can be excited by the 488-nm blue, 532-nm green, or 561-nm yellow-green laser and and transfers energy to the acceptor molecule, Cy7, which emits light that can be captured with a 780/60 nm bandpass filter. PE-CY7 has an excitation peak at 565 nm and an emission peak at 778 nm, and is a suitable alternative to PE-Vio®770 (Miltenyi) and cFluor BYG781 (Cytek).
Excitation & emission spectra
Key facts
- Excitation maximum
- 565 nm
- Emission maximum
- 778 nm
- Optimal laser line
- 488-561
- Optimal filter
- 780/60
- Relative brightness
- 4 of 5
- Extinction coefficient
- 1960000
- Molecular weight
- 240000
- Commercial antibodies
- 20.1k from 36 suppliers
PE-Cy7 has an excitation maximum at 565 nm and an emission maximum at 778 nm, a Stokes shift of 213 nm. Its extinction coefficient is 1960000 M⁻¹cm⁻¹. It is optimally excited by the 488-561 laser line.
Frequently asked questions
- What is PE-Cy7?
- PE-Cyanine®7 (PE-Cy7, RPE-Cy7) is a far red-emitting tandem fluorophore that combines phycoerythrin (PE) and Cy7. The donor molecule, PE can be excited by the 488-nm blue, 532-nm green, or 561-nm yellow-green laser and and transfers energy to the acceptor molecule, Cy7, which emits light that can be captured with a 780/60 nm bandpass filter. PE-CY7 has an excitation peak at 565 nm and an emission peak at 778 nm, and is a suitable alternative to PE-Vio®770 (Miltenyi) and cFluor BYG781 (Cytek).
- What laser excites PE-Cy7?
- PE-Cy7 is optimally excited by the 488-561 laser line. Its excitation maximum is 565 nm.
- What filter should I use to detect PE-Cy7?
- A 780/60 filter captures the peak of the PE-Cy7 emission near 778 nm with minimal spillover.
- How bright is PE-Cy7?
- PE-Cy7 has a relative brightness of 4 out of 5.
- How many antibodies are available conjugated to PE-Cy7?
- FluoroFinder tracks 20095 commercially available PE-Cy7 conjugates, searchable by target, clone, host and application.