Live/Dead Fix Near IR (775)
Live/Dead Fix Near IR (775) (Ex-Max 750 nm/Em-Max 775 nm)
The Live/Dead Fixable Viability probes from Thermo Fisher are amine-reactive and used for live/dead cell discrimination in flow cytometry. These probes pass through the permeable cell membrane of dead cells to stain intracellular amine groups. However, the probe cannot pass through the intact membranes of live cells. Additionally, these dyes are very stable and can withstand fixation, permeabilization, and will not transfer between cells. Live/Dead Fixable Near IR (775) has an excitation peak at 750 nm and an emission peak at 775 nm. In flow cytometry, the 633 nm laser is typically used for excitation.
Excitation & emission spectra
Key facts
- Excitation maximum
- 750 nm
- Emission maximum
- 775 nm
- Optimal laser line
- 628-640
- Optimal filter
- 780/60
- Commercial antibodies
- 3 from 1 supplier
Live/Dead Fix Near IR (775) has an excitation maximum at 750 nm and an emission maximum at 775 nm, a Stokes shift of 25 nm. It is optimally excited by the 628-640 laser line.
Frequently asked questions
- What is Live/Dead Fix Near IR (775)?
- The Live/Dead Fixable Viability probes from Thermo Fisher are amine-reactive and used for live/dead cell discrimination in flow cytometry. These probes pass through the permeable cell membrane of dead cells to stain intracellular amine groups. However, the probe cannot pass through the intact membranes of live cells. Additionally, these dyes are very stable and can withstand fixation, permeabilization, and will not transfer between cells. Live/Dead Fixable Near IR (775) has an excitation peak at 750 nm and an emission peak at 775 nm. In flow cytometry, the 633 nm laser is typically used for excitation.
- What laser excites Live/Dead Fix Near IR (775)?
- Live/Dead Fix Near IR (775) is optimally excited by the 628-640 laser line. Its excitation maximum is 750 nm.
- What filter should I use to detect Live/Dead Fix Near IR (775)?
- A 780/60 filter captures the peak of the Live/Dead Fix Near IR (775) emission near 775 nm with minimal spillover.
- How many antibodies are available conjugated to Live/Dead Fix Near IR (775)?
- FluoroFinder tracks 3 commercially available Live/Dead Fix Near IR (775) conjugates, searchable by target, clone, host and application.