Live/Dead Fix Far Red
Live/Dead Fix Far Red (Ex-Max 650 nm/Em-Max 670 nm)
The Live/Dead Fixable Viability probes from Thermo Fisher are amine-reactive and used for live/dead cell discrimination in flow cytometry. These probes pass through the permeable cell membrane of dead cells to stain intracellular amine groups. However, the probe cannot pass through the intact membranes of live cells. Additionally, these dyes are very stable and can withstand fixation, permeabilization, and will not transfer between cells. Live/Dead Fixable Far Red has an excitation peak at 650 nm and an emission peak at 670 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 650 nm
- Emission maximum
- 670 nm
- Optimal laser line
- 628-640
- Optimal filter
- 660/20
- Commercial antibodies
- 3 from 1 supplier
Live/Dead Fix Far Red has an excitation maximum at 650 nm and an emission maximum at 670 nm, a Stokes shift of 20 nm. It is optimally excited by the 628-640 laser line.
Frequently asked questions
- What is Live/Dead Fix Far Red?
- The Live/Dead Fixable Viability probes from Thermo Fisher are amine-reactive and used for live/dead cell discrimination in flow cytometry. These probes pass through the permeable cell membrane of dead cells to stain intracellular amine groups. However, the probe cannot pass through the intact membranes of live cells. Additionally, these dyes are very stable and can withstand fixation, permeabilization, and will not transfer between cells. Live/Dead Fixable Far Red has an excitation peak at 650 nm and an emission peak at 670 nm.
- What laser excites Live/Dead Fix Far Red?
- Live/Dead Fix Far Red is optimally excited by the 628-640 laser line. Its excitation maximum is 650 nm.
- What filter should I use to detect Live/Dead Fix Far Red?
- A 660/20 filter captures the peak of the Live/Dead Fix Far Red emission near 670 nm with minimal spillover.
- How many antibodies are available conjugated to Live/Dead Fix Far Red?
- FluoroFinder tracks 3 commercially available Live/Dead Fix Far Red conjugates, searchable by target, clone, host and application.