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PE-Alexa 647

PE-Alexa 647 (Ex-Max 565 nm/Em-Max 668 nm)

PE-Alexa 647 from Thermo Fisher Scientific has an excitation peak at 565 nm and an emission peak at 668 nm. It is useful for flow cytometry applications. PE-Alexa 647 is an alternative to PE-Cy5.

Excitation & emission spectra

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Key facts

Excitation maximum
565 nm
Emission maximum
668 nm
Optimal laser line
488-561
Optimal filter
660/20
Relative brightness
3 of 5
Commercial antibodies
7 from 2 suppliers

PE-Alexa 647 has an excitation maximum at 565 nm and an emission maximum at 668 nm, a Stokes shift of 103 nm. It is optimally excited by the 488-561 laser line.

Frequently asked questions

What is PE-Alexa 647?
PE-Alexa 647 from Thermo Fisher Scientific has an excitation peak at 565 nm and an emission peak at 668 nm. It is useful for flow cytometry applications. PE-Alexa 647 is an alternative to PE-Cy5.
What laser excites PE-Alexa 647?
PE-Alexa 647 is optimally excited by the 488-561 laser line. Its excitation maximum is 565 nm.
What filter should I use to detect PE-Alexa 647?
A 660/20 filter captures the peak of the PE-Alexa 647 emission near 668 nm with minimal spillover.
How bright is PE-Alexa 647?
PE-Alexa 647 has a relative brightness of 3 out of 5.
How many antibodies are available conjugated to PE-Alexa 647?
FluoroFinder tracks 7 commercially available PE-Alexa 647 conjugates, searchable by target, clone, host and application.