Live/Dead Fix Green
Live/Dead Fix Green (Ex-Max 496 nm/Em-Max 523 nm)
The Live/Dead Fixable Viability probes from Thermo Fisher are amine-reactive and used for live/dead cell discrimination in flow cytometry. These probes pass through the permeable cell membrane of dead cells to stain intracellular amine groups. However, the probe cannot pass through the intact membranes of live cells. Additionally, these dyes are very stable and can withstand fixation, permeabilization, and will not transfer between cells. Live/Dead Fixable Green has an excitation peak at 496 nm and an emission peak at 523 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 496 nm
- Emission maximum
- 523 nm
- Optimal laser line
- 488
- Optimal filter
- 515/30
- Commercial antibodies
- 3 from 1 supplier
Live/Dead Fix Green has an excitation maximum at 496 nm and an emission maximum at 523 nm, a Stokes shift of 27 nm. It is optimally excited by the 488 laser line.
Frequently asked questions
- What is Live/Dead Fix Green?
- The Live/Dead Fixable Viability probes from Thermo Fisher are amine-reactive and used for live/dead cell discrimination in flow cytometry. These probes pass through the permeable cell membrane of dead cells to stain intracellular amine groups. However, the probe cannot pass through the intact membranes of live cells. Additionally, these dyes are very stable and can withstand fixation, permeabilization, and will not transfer between cells. Live/Dead Fixable Green has an excitation peak at 496 nm and an emission peak at 523 nm.
- What laser excites Live/Dead Fix Green?
- Live/Dead Fix Green is optimally excited by the 488 laser line. Its excitation maximum is 496 nm.
- What filter should I use to detect Live/Dead Fix Green?
- A 515/30 filter captures the peak of the Live/Dead Fix Green emission near 523 nm with minimal spillover.
- How many antibodies are available conjugated to Live/Dead Fix Green?
- FluoroFinder tracks 3 commercially available Live/Dead Fix Green conjugates, searchable by target, clone, host and application.