Live/Dead Fix Yellow
Live/Dead Fix Yellow (Ex-Max 400 nm/Em-Max 550 nm)
The Live/Dead Fixable Viability probes from Thermo Fisher are amine-reactive and used for live/dead cell discrimination in flow cytometry. These probes pass through the permeable cell membrane of dead cells to stain intracellular amine groups. However, the probe cannot pass through the intact membranes of live cells. Additionally, these dyes are very stable and can withstand fixation, permeabilization, and will not transfer between cells. Live/Dead Fixable Yellow has an excitation peak at 400 nm and an emission peak at 550 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 400 nm
- Emission maximum
- 550 nm
- Optimal laser line
- 405
- Optimal filter
- 550/40
- Commercial antibodies
- 3 from 1 supplier
Live/Dead Fix Yellow has an excitation maximum at 400 nm and an emission maximum at 550 nm, a Stokes shift of 150 nm. It is optimally excited by the 405 laser line.
Frequently asked questions
- What is Live/Dead Fix Yellow?
- The Live/Dead Fixable Viability probes from Thermo Fisher are amine-reactive and used for live/dead cell discrimination in flow cytometry. These probes pass through the permeable cell membrane of dead cells to stain intracellular amine groups. However, the probe cannot pass through the intact membranes of live cells. Additionally, these dyes are very stable and can withstand fixation, permeabilization, and will not transfer between cells. Live/Dead Fixable Yellow has an excitation peak at 400 nm and an emission peak at 550 nm.
- What laser excites Live/Dead Fix Yellow?
- Live/Dead Fix Yellow is optimally excited by the 405 laser line. Its excitation maximum is 400 nm.
- What filter should I use to detect Live/Dead Fix Yellow?
- A 550/40 filter captures the peak of the Live/Dead Fix Yellow emission near 550 nm with minimal spillover.
- How many antibodies are available conjugated to Live/Dead Fix Yellow?
- FluoroFinder tracks 3 commercially available Live/Dead Fix Yellow conjugates, searchable by target, clone, host and application.