Live/Dead Fix Violet
Live/Dead Fix Violet (Ex-Max 402 nm/Em-Max 452 nm)
The Live/Dead Fixable Viability probes from Thermo Fisher are amine-reactive and used for live/dead cell discrimination in flow cytometry. These probes pass through the permeable cell membrane of dead cells to stain intracellular amine groups. However, the probe cannot pass through the intact membranes of live cells. Additionally, these dyes are very stable and can withstand fixation, permeabilization, and will not transfer between cells. Live/Dead Fixable Violet has an excitation peak at 402 nm and an emission peak at 452 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 402 nm
- Emission maximum
- 452 nm
- Optimal laser line
- 405
- Optimal filter
- 450/50
- Commercial antibodies
- 4 from 1 supplier
Live/Dead Fix Violet has an excitation maximum at 402 nm and an emission maximum at 452 nm, a Stokes shift of 50 nm. It is optimally excited by the 405 laser line.
Frequently asked questions
- What is Live/Dead Fix Violet?
- The Live/Dead Fixable Viability probes from Thermo Fisher are amine-reactive and used for live/dead cell discrimination in flow cytometry. These probes pass through the permeable cell membrane of dead cells to stain intracellular amine groups. However, the probe cannot pass through the intact membranes of live cells. Additionally, these dyes are very stable and can withstand fixation, permeabilization, and will not transfer between cells. Live/Dead Fixable Violet has an excitation peak at 402 nm and an emission peak at 452 nm.
- What laser excites Live/Dead Fix Violet?
- Live/Dead Fix Violet is optimally excited by the 405 laser line. Its excitation maximum is 402 nm.
- What filter should I use to detect Live/Dead Fix Violet?
- A 450/50 filter captures the peak of the Live/Dead Fix Violet emission near 452 nm with minimal spillover.
- How many antibodies are available conjugated to Live/Dead Fix Violet?
- FluoroFinder tracks 4 commercially available Live/Dead Fix Violet conjugates, searchable by target, clone, host and application.