PE
PE (Ex-Max 565 nm/Em-Max 578 nm)
Phycoerythrin (PE, R-PE) is a red-emitting fluorescent protein-chromophore complex that can be excited the 488-nm blue, 532-nm green, or 561-nm yellow-green laser with increasing efficiency and captured with a 586/14 nm bandpass filter. PE has an excitation peak at 565 nm and an emission peak at 578 nm. PE is 240kD in size and has an extinction coefficient of ~2x10^6 which makes it one of the brightest fluorophores available and a potent donor upon which to build tandem fluorophores with longer Stoke's Shifts.
Excitation & emission spectra
Key facts
- Excitation maximum
- 565 nm
- Emission maximum
- 578 nm
- Optimal laser line
- 488-561
- Optimal filter
- 586/14
- Relative brightness
- 5 of 5
- Extinction coefficient
- 1960000
- Quantum yield
- 0.84
- Molecular weight
- 240000
- Commercial antibodies
- 130k from 62 suppliers
PE has an excitation maximum at 565 nm and an emission maximum at 578 nm, a Stokes shift of 13 nm. Its extinction coefficient is 1960000 M⁻¹cm⁻¹. It is optimally excited by the 488-561 laser line.
Frequently asked questions
- What is PE?
- Phycoerythrin (PE, R-PE) is a red-emitting fluorescent protein-chromophore complex that can be excited the 488-nm blue, 532-nm green, or 561-nm yellow-green laser with increasing efficiency and captured with a 586/14 nm bandpass filter. PE has an excitation peak at 565 nm and an emission peak at 578 nm. PE is 240kD in size and has an extinction coefficient of ~2x10^6 which makes it one of the brightest fluorophores available and a potent donor upon which to build tandem fluorophores with longer Stoke's Shifts.
- What laser excites PE?
- PE is optimally excited by the 488-561 laser line. Its excitation maximum is 565 nm.
- What filter should I use to detect PE?
- A 586/14 filter captures the peak of the PE emission near 578 nm with minimal spillover.
- How bright is PE?
- PE has a relative brightness of 5 out of 5 (quantum yield 0.84).
- How many antibodies are available conjugated to PE?
- FluoroFinder tracks 130030 commercially available PE conjugates, searchable by target, clone, host and application.