RealViolet 544
RV544
RealViolet 544 is a fluorescent dye produced by Waters Biosciences (formerly BD Biosciences) intended for use in spectral flow cytometry. It's excited by the violet laser (ex peak at 412 nm), and peak emission is 546 nm. Its moderate brightness makes it good for medium or high expression surface and intracellular markers.
Excitation & emission spectra
Key facts
- Excitation maximum
- 412 nm
- Emission maximum
- 546 nm
- Optimal laser line
- 405
- Relative brightness
- 2 of 5
- Commercial antibodies
- 48 from 1 supplier
RealViolet 544 has an excitation maximum at 412 nm and an emission maximum at 546 nm, a Stokes shift of 134 nm. It is optimally excited by the 405 laser line.
Frequently asked questions
- What is RealViolet 544?
- RealViolet 544 is a fluorescent dye produced by Waters Biosciences (formerly BD Biosciences) intended for use in spectral flow cytometry. It's excited by the violet laser (ex peak at 412 nm), and peak emission is 546 nm. Its moderate brightness makes it good for medium or high expression surface and intracellular markers.
- What laser excites RealViolet 544?
- RealViolet 544 is optimally excited by the 405 laser line. Its excitation maximum is 412 nm.
- How bright is RealViolet 544?
- RealViolet 544 has a relative brightness of 2 out of 5.
- How many antibodies are available conjugated to RealViolet 544?
- FluoroFinder tracks 48 commercially available RealViolet 544 conjugates, searchable by target, clone, host and application.