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Calcofluor White

Calcofluor White is a fluorescent blue dye that binds to cellulose and chitin in the cell walls of fungi, plants, and algae. Itis used in clinical mycology for the rapid identification of fungal infections in biopsy samples, and in biology to visualize cell wall dynamics.

Excitation & emission spectra

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Key facts

Excitation maximum
350 nm
Emission maximum
431 nm
Optimal laser line
355

Calcofluor White has an excitation maximum at 350 nm and an emission maximum at 431 nm, a Stokes shift of 81 nm. It is optimally excited by the 355 laser line.

Frequently asked questions

What is Calcofluor White?
Calcofluor White is a fluorescent blue dye that binds to cellulose and chitin in the cell walls of fungi, plants, and algae. Itis used in clinical mycology for the rapid identification of fungal infections in biopsy samples, and in biology to visualize cell wall dynamics.
What laser excites Calcofluor White?
Calcofluor White is optimally excited by the 355 laser line. Its excitation maximum is 350 nm.