Spark PLUS V450
Spark PLUS V450™ from Biolegend is a fluorophore designed for both conventional and spectral flow cytometry with an excitation peak at 405 nm and an emission peak at 452 nm. This fluorophore is most efficiently excited by the 405 nm laser and is very bright, making it very versatile.
Excitation & emission spectra
Key facts
- Excitation maximum
- 405 nm
- Emission maximum
- 452 nm
- Optimal laser line
- 405
- Optimal filter
- 450/50
- Relative brightness
- 4 of 5
- Commercial antibodies
- 44 from 2 suppliers
Spark PLUS V450 has an excitation maximum at 405 nm and an emission maximum at 452 nm, a Stokes shift of 47 nm. It is optimally excited by the 405 laser line.
Frequently asked questions
- What is Spark PLUS V450?
- Spark PLUS V450™ from Biolegend is a fluorophore designed for both conventional and spectral flow cytometry with an excitation peak at 405 nm and an emission peak at 452 nm. This fluorophore is most efficiently excited by the 405 nm laser and is very bright, making it very versatile.
- What laser excites Spark PLUS V450?
- Spark PLUS V450 is optimally excited by the 405 laser line. Its excitation maximum is 405 nm.
- What filter should I use to detect Spark PLUS V450?
- A 450/50 filter captures the peak of the Spark PLUS V450 emission near 452 nm with minimal spillover.
- How bright is Spark PLUS V450?
- Spark PLUS V450 has a relative brightness of 4 out of 5.
- How many antibodies are available conjugated to Spark PLUS V450?
- FluoroFinder tracks 44 commercially available Spark PLUS V450 conjugates, searchable by target, clone, host and application.