NovaFluor Blue 745
NF-B745
The NovaFluor family of fluorescent polymers from Thermo Fisher Scientific are a novel reagent for traditional and spectral flow cytometry. These fluorophores are comprised of a DNA backbone polymer integrated with fluorophore substituted nucleosides to create a potent donor molecule. Tandem fluophores with long Stokes' Shift enable brighter, spectrally discrete, tunable fluorescent polymers as an alternative to traditional simple organic structures, protein-based fluorophores as well as the Horizon Brilliant™ family of fluorescent polymers from BD. NovaFluor Blue 745 has an excitation peak at 492 nm and an emission peak at 750 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 492 nm
- Emission maximum
- 750 nm
- Optimal laser line
- 488
- Optimal filter
- 738/23
- Relative brightness
- 2 of 5
- Commercial antibodies
- 18 from 1 supplier
NovaFluor Blue 745 has an excitation maximum at 492 nm and an emission maximum at 750 nm, a Stokes shift of 258 nm. It is optimally excited by the 488 laser line.
Frequently asked questions
- What is NovaFluor Blue 745?
- The NovaFluor family of fluorescent polymers from Thermo Fisher Scientific are a novel reagent for traditional and spectral flow cytometry. These fluorophores are comprised of a DNA backbone polymer integrated with fluorophore substituted nucleosides to create a potent donor molecule. Tandem fluophores with long Stokes' Shift enable brighter, spectrally discrete, tunable fluorescent polymers as an alternative to traditional simple organic structures, protein-based fluorophores as well as the Horizon Brilliant™ family of fluorescent polymers from BD. NovaFluor Blue 745 has an excitation peak at 492 nm and an emission peak at 750 nm.
- What laser excites NovaFluor Blue 745?
- NovaFluor Blue 745 is optimally excited by the 488 laser line. Its excitation maximum is 492 nm.
- What filter should I use to detect NovaFluor Blue 745?
- A 738/23 filter captures the peak of the NovaFluor Blue 745 emission near 750 nm with minimal spillover.
- How bright is NovaFluor Blue 745?
- NovaFluor Blue 745 has a relative brightness of 2 out of 5.
- How many antibodies are available conjugated to NovaFluor Blue 745?
- FluoroFinder tracks 18 commercially available NovaFluor Blue 745 conjugates, searchable by target, clone, host and application.