Live-or-Dye 700/745
Biotium's Live-or-Dye™ dyes are amine-reactive and used for live/dead cell discrimination in flow cytometry. These probes pass through the permeable cell membrane of dead cells to stain intracellular amine groups. However, the probe cannot pass through the intact membranes of live cells. Additionally, these dyes are very stable and can withstand fixation, permeabilization, and will not transfer between cells. Live-or-Dye™ 700/745 has an excitation peak at 700 nm and an emission peak at 725 nm. In flow cytometry, the 633nm laser is typically used for excitation.
Excitation & emission spectra
Key facts
- Excitation maximum
- 700 nm
- Emission maximum
- 725 nm
- Optimal laser line
- 633-640, 785
- Commercial antibodies
- 2 from 1 supplier
Live-or-Dye 700/745 has an excitation maximum at 700 nm and an emission maximum at 725 nm, a Stokes shift of 25 nm. It is optimally excited by the 633-640, 785 laser line.
Frequently asked questions
- What is Live-or-Dye 700/745?
- Biotium's Live-or-Dye™ dyes are amine-reactive and used for live/dead cell discrimination in flow cytometry. These probes pass through the permeable cell membrane of dead cells to stain intracellular amine groups. However, the probe cannot pass through the intact membranes of live cells. Additionally, these dyes are very stable and can withstand fixation, permeabilization, and will not transfer between cells. Live-or-Dye™ 700/745 has an excitation peak at 700 nm and an emission peak at 725 nm. In flow cytometry, the 633nm laser is typically used for excitation.
- What laser excites Live-or-Dye 700/745?
- Live-or-Dye 700/745 is optimally excited by the 633-640, 785 laser line. Its excitation maximum is 700 nm.
- How many antibodies are available conjugated to Live-or-Dye 700/745?
- FluoroFinder tracks 2 commercially available Live-or-Dye 700/745 conjugates, searchable by target, clone, host and application.