Live-or-Dye 650/720
Biotium's Live-or-Dye™ dyes are amine-reactive and used for live/dead cell discrimination in flow cytometry. These probes pass through the permeable cell membrane of dead cells to stain intracellular amine groups. However, the probe cannot pass through the intact membranes of live cells. Additionally, these dyes are very stable and can withstand fixation, permeabilization, and will not transfer between cells. Live-or-Dye™ 650/720 has an excitation peak at 650 nm and an emission peak at 720 nm. In flow cytometry, the 633nm laser is typically used for excitation.
Excitation & emission spectra
Key facts
- Excitation maximum
- 650 nm
- Emission maximum
- 720 nm
- Optimal laser line
- 633-640
- Commercial antibodies
- 2 from 1 supplier
Live-or-Dye 650/720 has an excitation maximum at 650 nm and an emission maximum at 720 nm, a Stokes shift of 70 nm. It is optimally excited by the 633-640 laser line.
Frequently asked questions
- What is Live-or-Dye 650/720?
- Biotium's Live-or-Dye™ dyes are amine-reactive and used for live/dead cell discrimination in flow cytometry. These probes pass through the permeable cell membrane of dead cells to stain intracellular amine groups. However, the probe cannot pass through the intact membranes of live cells. Additionally, these dyes are very stable and can withstand fixation, permeabilization, and will not transfer between cells. Live-or-Dye™ 650/720 has an excitation peak at 650 nm and an emission peak at 720 nm. In flow cytometry, the 633nm laser is typically used for excitation.
- What laser excites Live-or-Dye 650/720?
- Live-or-Dye 650/720 is optimally excited by the 633-640 laser line. Its excitation maximum is 650 nm.
- How many antibodies are available conjugated to Live-or-Dye 650/720?
- FluoroFinder tracks 2 commercially available Live-or-Dye 650/720 conjugates, searchable by target, clone, host and application.