Live-or-Dye 850/870
Biotium's Live-or-Dye™ dyes are amine-reactive and used for live/dead cell discrimination in flow cytometry. These probes pass through the permeable cell membrane of dead cells to stain intracellular amine groups. However, the probe cannot pass through the intact membranes of live cells. Additionally, these dyes are very stable and can withstand fixation, permeabilization, and will not transfer between cells. Live-or-Dye™ 850/870 has an excitation peak at 852 nm and an emission peak at 870 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 852 nm
- Emission maximum
- 870 nm
- Optimal laser line
- 808
- Commercial antibodies
- 2 from 1 supplier
Live-or-Dye 850/870 has an excitation maximum at 852 nm and an emission maximum at 870 nm, a Stokes shift of 18 nm. It is optimally excited by the 808 laser line.
Frequently asked questions
- What is Live-or-Dye 850/870?
- Biotium's Live-or-Dye™ dyes are amine-reactive and used for live/dead cell discrimination in flow cytometry. These probes pass through the permeable cell membrane of dead cells to stain intracellular amine groups. However, the probe cannot pass through the intact membranes of live cells. Additionally, these dyes are very stable and can withstand fixation, permeabilization, and will not transfer between cells. Live-or-Dye™ 850/870 has an excitation peak at 852 nm and an emission peak at 870 nm.
- What laser excites Live-or-Dye 850/870?
- Live-or-Dye 850/870 is optimally excited by the 808 laser line. Its excitation maximum is 852 nm.
- How many antibodies are available conjugated to Live-or-Dye 850/870?
- FluoroFinder tracks 2 commercially available Live-or-Dye 850/870 conjugates, searchable by target, clone, host and application.