Live-or-Dye 820/835
Biotium's Live-or-Dye™ dyes are amine-reactive and used for live/dead cell discrimination in flow cytometry. These probes pass through the permeable cell membrane of dead cells to stain intracellular amine groups. However, the probe cannot pass through the intact membranes of live cells. Additionally, these dyes are very stable and can withstand fixation, permeabilization, and will not transfer between cells. Live-or-Dye™ 820/835 has an excitation peak at 822 nm and an emission peak at 835 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 822 nm
- Emission maximum
- 835 nm
- Optimal laser line
- 808
- Commercial antibodies
- 2 from 1 supplier
Live-or-Dye 820/835 has an excitation maximum at 822 nm and an emission maximum at 835 nm, a Stokes shift of 13 nm. It is optimally excited by the 808 laser line.
Frequently asked questions
- What is Live-or-Dye 820/835?
- Biotium's Live-or-Dye™ dyes are amine-reactive and used for live/dead cell discrimination in flow cytometry. These probes pass through the permeable cell membrane of dead cells to stain intracellular amine groups. However, the probe cannot pass through the intact membranes of live cells. Additionally, these dyes are very stable and can withstand fixation, permeabilization, and will not transfer between cells. Live-or-Dye™ 820/835 has an excitation peak at 822 nm and an emission peak at 835 nm.
- What laser excites Live-or-Dye 820/835?
- Live-or-Dye 820/835 is optimally excited by the 808 laser line. Its excitation maximum is 822 nm.
- How many antibodies are available conjugated to Live-or-Dye 820/835?
- FluoroFinder tracks 2 commercially available Live-or-Dye 820/835 conjugates, searchable by target, clone, host and application.