Spark PLUS B574
Spark PLUS B574™ from Biolegend is a fluorophore for use in both conventional and spectral flow cytometry with an excitation peak at 506 nm and an emission peak at 574 nm. While it’s suitable for both conventional and spectral flow, it demonstrates maximum brightness on spectral cytometers. It can be unmixed from colors like Spark PLUS B550™, FITC, and PerCP/Cyanine5.5, which makes it a good option for large spectral panels. It can be unmixed from PE on instruments with both blue and yellow/green lasers.
Excitation & emission spectra
Key facts
- Excitation maximum
- 505 nm
- Emission maximum
- 574 nm
- Optimal laser line
- 488
- Relative brightness
- 4 of 5
- Commercial antibodies
- 40 from 2 suppliers
Spark PLUS B574 has an excitation maximum at 505 nm and an emission maximum at 574 nm, a Stokes shift of 69 nm. It is optimally excited by the 488 laser line.
Frequently asked questions
- What is Spark PLUS B574?
- Spark PLUS B574™ from Biolegend is a fluorophore for use in both conventional and spectral flow cytometry with an excitation peak at 506 nm and an emission peak at 574 nm. While it’s suitable for both conventional and spectral flow, it demonstrates maximum brightness on spectral cytometers. It can be unmixed from colors like Spark PLUS B550™, FITC, and PerCP/Cyanine5.5, which makes it a good option for large spectral panels. It can be unmixed from PE on instruments with both blue and yellow/green lasers.
- What laser excites Spark PLUS B574?
- Spark PLUS B574 is optimally excited by the 488 laser line. Its excitation maximum is 505 nm.
- How bright is Spark PLUS B574?
- Spark PLUS B574 has a relative brightness of 4 out of 5.
- How many antibodies are available conjugated to Spark PLUS B574?
- FluoroFinder tracks 40 commercially available Spark PLUS B574 conjugates, searchable by target, clone, host and application.