Spark PLUS V475
Spark PLUS V475™ has an excitation peak at 404 nm and an emission peak at 475 nm. Spark PLUS V475™ provides a bright, cleaner alternative to Brilliant Violet 480™ with reduced spillover into Brilliant Violet 510™. Spark PLUS V475™ can be unmixed from other violet laser options and can replace Brilliant Violet 480™ on conventional instruments, while its unique spectral profile also allows clear unmixing from BV480™ on spectral instruments. Spark PLUS Dyes employ proprietary technology to maximize signal and boost performance while maintaining the advantages of Spark Dyes, such as high stability and buffer compatibility.
Excitation & emission spectra
Key facts
- Excitation maximum
- 404 nm
- Emission maximum
- 475 nm
- Optimal laser line
- 405
- Relative brightness
- 4 of 5
- Commercial antibodies
- 69 from 2 suppliers
Spark PLUS V475 has an excitation maximum at 404 nm and an emission maximum at 475 nm, a Stokes shift of 71 nm. It is optimally excited by the 405 laser line.
Frequently asked questions
- What is Spark PLUS V475?
- Spark PLUS V475™ has an excitation peak at 404 nm and an emission peak at 475 nm. Spark PLUS V475™ provides a bright, cleaner alternative to Brilliant Violet 480™ with reduced spillover into Brilliant Violet 510™. Spark PLUS V475™ can be unmixed from other violet laser options and can replace Brilliant Violet 480™ on conventional instruments, while its unique spectral profile also allows clear unmixing from BV480™ on spectral instruments. Spark PLUS Dyes employ proprietary technology to maximize signal and boost performance while maintaining the advantages of Spark Dyes, such as high stability and buffer compatibility.
- What laser excites Spark PLUS V475?
- Spark PLUS V475 is optimally excited by the 405 laser line. Its excitation maximum is 404 nm.
- How bright is Spark PLUS V475?
- Spark PLUS V475 has a relative brightness of 4 out of 5.
- How many antibodies are available conjugated to Spark PLUS V475?
- FluoroFinder tracks 69 commercially available Spark PLUS V475 conjugates, searchable by target, clone, host and application.