RealYellow 743
RY743
BD Horizon RealYellow 743 (RY743) has an excitation peak at 557 nm and an emission peak at 743 nm. RY743 reagents offer a bright fluorochrome to support the detection of low expression surface and intracellular markers and increase panel design flexibility, offering minimal cross-laser excitation from the 488-nm blue laser in hundreds of antibody specificities.. RY743 is excited primarily by the 561 nm yellow-green laser.
Excitation & emission spectra
Key facts
- Excitation maximum
- 557 nm
- Emission maximum
- 743 nm
- Optimal laser line
- 561
- Optimal filter
- 750/30
- Relative brightness
- 4 of 5
- Commercial antibodies
- 770 from 1 supplier
RealYellow 743 has an excitation maximum at 557 nm and an emission maximum at 743 nm, a Stokes shift of 186 nm. It is optimally excited by the 561 laser line.
Frequently asked questions
- What is RealYellow 743?
- BD Horizon RealYellow 743 (RY743) has an excitation peak at 557 nm and an emission peak at 743 nm. RY743 reagents offer a bright fluorochrome to support the detection of low expression surface and intracellular markers and increase panel design flexibility, offering minimal cross-laser excitation from the 488-nm blue laser in hundreds of antibody specificities.. RY743 is excited primarily by the 561 nm yellow-green laser.
- What laser excites RealYellow 743?
- RealYellow 743 is optimally excited by the 561 laser line. Its excitation maximum is 557 nm.
- What filter should I use to detect RealYellow 743?
- A 750/30 filter captures the peak of the RealYellow 743 emission near 743 nm with minimal spillover.
- How bright is RealYellow 743?
- RealYellow 743 has a relative brightness of 4 out of 5.
- How many antibodies are available conjugated to RealYellow 743?
- FluoroFinder tracks 770 commercially available RealYellow 743 conjugates, searchable by target, clone, host and application.