RealYellow 655
RY655
BD Horizon RealYellow 655 (RY655) has an excitation peak at 557 nm and an emission peak at 654 nm. RY655 reagents offer a bright fluorochrome to support the detection of low expression surface and intracellular markers and increase panel design flexibility, offering minimal cross-laser excitation from the 488-nm blue laser in hundreds of antibody specificities.. RY655 is excited primarily by the 561 nm yellow-green laser.
Excitation & emission spectra
Key facts
- Excitation maximum
- 557 nm
- Emission maximum
- 654 nm
- Optimal laser line
- 561
- Optimal filter
- 660/30
- Relative brightness
- 5 of 5
- Commercial antibodies
- 815 from 1 supplier
RealYellow 655 has an excitation maximum at 557 nm and an emission maximum at 654 nm, a Stokes shift of 97 nm. It is optimally excited by the 561 laser line.
Frequently asked questions
- What is RealYellow 655?
- BD Horizon RealYellow 655 (RY655) has an excitation peak at 557 nm and an emission peak at 654 nm. RY655 reagents offer a bright fluorochrome to support the detection of low expression surface and intracellular markers and increase panel design flexibility, offering minimal cross-laser excitation from the 488-nm blue laser in hundreds of antibody specificities.. RY655 is excited primarily by the 561 nm yellow-green laser.
- What laser excites RealYellow 655?
- RealYellow 655 is optimally excited by the 561 laser line. Its excitation maximum is 557 nm.
- What filter should I use to detect RealYellow 655?
- A 660/30 filter captures the peak of the RealYellow 655 emission near 654 nm with minimal spillover.
- How bright is RealYellow 655?
- RealYellow 655 has a relative brightness of 5 out of 5.
- How many antibodies are available conjugated to RealYellow 655?
- FluoroFinder tracks 815 commercially available RealYellow 655 conjugates, searchable by target, clone, host and application.