Spark PLUS B550
Spark PLUS B550 has an excitation peak at 516 nm and an emission peak at 540 nm. Spark PLUS B550 is highly resistant to heat, light, and common fixatives. Spark PLUS B550 can be used on conventional and spectral flow cytometers, and is ideal for combining with PE and FITC on spectral instruments. Spark PLUS B550 maximizes signal from the blue laser with minimal yellow/green cross-excitation and spillover.
Excitation & emission spectra
Key facts
- Excitation maximum
- 516 nm
- Emission maximum
- 540 nm
- Optimal laser line
- 488
- Optimal filter
- 525/50
- Relative brightness
- 3 of 5
- Commercial antibodies
- 162 from 2 suppliers
Spark PLUS B550 has an excitation maximum at 516 nm and an emission maximum at 540 nm, a Stokes shift of 24 nm. It is optimally excited by the 488 laser line.
Frequently asked questions
- What is Spark PLUS B550?
- Spark PLUS B550 has an excitation peak at 516 nm and an emission peak at 540 nm. Spark PLUS B550 is highly resistant to heat, light, and common fixatives. Spark PLUS B550 can be used on conventional and spectral flow cytometers, and is ideal for combining with PE and FITC on spectral instruments. Spark PLUS B550 maximizes signal from the blue laser with minimal yellow/green cross-excitation and spillover.
- What laser excites Spark PLUS B550?
- Spark PLUS B550 is optimally excited by the 488 laser line. Its excitation maximum is 516 nm.
- What filter should I use to detect Spark PLUS B550?
- A 525/50 filter captures the peak of the Spark PLUS B550 emission near 540 nm with minimal spillover.
- How bright is Spark PLUS B550?
- Spark PLUS B550 has a relative brightness of 3 out of 5.
- How many antibodies are available conjugated to Spark PLUS B550?
- FluoroFinder tracks 162 commercially available Spark PLUS B550 conjugates, searchable by target, clone, host and application.