RealYellow 775
RY775
RealYellow™ 775 (RY775) from BD Biosciences is an alternative to PE-Cy7, PE-Vio 770 (Miltenyi) and PE-eFluor 780 (ThermoFisher) tandem fluorophores and has the advantage of discrete excitation off of the 561 nm laser without much cross-beam excitation by the 488nm laser. This limited cross-beam excitation will translate to lower complexity index scores and lower spillover spreading error in high parameter spectral flow cytometry applications.
Excitation & emission spectra
Key facts
- Excitation maximum
- 557 nm
- Emission maximum
- 775 nm
- Optimal laser line
- 561
- Relative brightness
- 5 of 5
- Commercial antibodies
- 849 from 1 supplier
RealYellow 775 has an excitation maximum at 557 nm and an emission maximum at 775 nm, a Stokes shift of 218 nm. It is optimally excited by the 561 laser line.
Frequently asked questions
- What is RealYellow 775?
- RealYellow™ 775 (RY775) from BD Biosciences is an alternative to PE-Cy7, PE-Vio 770 (Miltenyi) and PE-eFluor 780 (ThermoFisher) tandem fluorophores and has the advantage of discrete excitation off of the 561 nm laser without much cross-beam excitation by the 488nm laser. This limited cross-beam excitation will translate to lower complexity index scores and lower spillover spreading error in high parameter spectral flow cytometry applications.
- What laser excites RealYellow 775?
- RealYellow 775 is optimally excited by the 561 laser line. Its excitation maximum is 557 nm.
- How bright is RealYellow 775?
- RealYellow 775 has a relative brightness of 5 out of 5.
- How many antibodies are available conjugated to RealYellow 775?
- FluoroFinder tracks 849 commercially available RealYellow 775 conjugates, searchable by target, clone, host and application.