RealYellow 703
RY703
RealYellow 703 (RY703) is a spectrally novel fluorophore from BD Biosciences. The closest spectral equivalent is PE-Cy5.5 and PE-Fire 700 (BioLegend) but is far superior in its discrete excitation off the 561nm laser and limited cross-beam excitation by the 488nm laser. This limited cross-beam excitation will translate to lower complexity index scores and lower spillover spreading error in high parameter spectral flow cytometry applications.
Excitation & emission spectra
Key facts
- Excitation maximum
- 557 nm
- Emission maximum
- 703 nm
- Optimal laser line
- 561
- Relative brightness
- 5 of 5
- Commercial antibodies
- 860 from 1 supplier
RealYellow 703 has an excitation maximum at 557 nm and an emission maximum at 703 nm, a Stokes shift of 146 nm. It is optimally excited by the 561 laser line.
Frequently asked questions
- What is RealYellow 703?
- RealYellow 703 (RY703) is a spectrally novel fluorophore from BD Biosciences. The closest spectral equivalent is PE-Cy5.5 and PE-Fire 700 (BioLegend) but is far superior in its discrete excitation off the 561nm laser and limited cross-beam excitation by the 488nm laser. This limited cross-beam excitation will translate to lower complexity index scores and lower spillover spreading error in high parameter spectral flow cytometry applications.
- What laser excites RealYellow 703?
- RealYellow 703 is optimally excited by the 561 laser line. Its excitation maximum is 557 nm.
- How bright is RealYellow 703?
- RealYellow 703 has a relative brightness of 5 out of 5.
- How many antibodies are available conjugated to RealYellow 703?
- FluoroFinder tracks 860 commercially available RealYellow 703 conjugates, searchable by target, clone, host and application.