RayBright PerCP
Peridinin-Chlorophyll-Protein (PerCP) is a red-emitting fluorescent protein isolated from algae that can be excited by the 488 nm blue laser and captured with a 670/30 nm bandpass filter. PerCP exhibits a large Stokes' Shift, with an excitation peak at 482 nm and an emission peak at 675 nm. PerCP is was historically used in flow cytometry, however it is highly susceptible to photobleaching and has poor stability. Alternatives like RealBlue 705 (BD Biosciences), NovaFluor Blue 690 or PerCP-eFluor™ 710 are preferred.
Excitation & emission spectra
Key facts
- Excitation maximum
- 482 nm
- Emission maximum
- 675 nm
- Optimal laser line
- 488
- Relative brightness
- 1 of 5
- Commercial antibodies
- 34 from 1 supplier
RayBright PerCP has an excitation maximum at 482 nm and an emission maximum at 675 nm, a Stokes shift of 193 nm. It is optimally excited by the 488 laser line.
Frequently asked questions
- What is RayBright PerCP?
- Peridinin-Chlorophyll-Protein (PerCP) is a red-emitting fluorescent protein isolated from algae that can be excited by the 488 nm blue laser and captured with a 670/30 nm bandpass filter. PerCP exhibits a large Stokes' Shift, with an excitation peak at 482 nm and an emission peak at 675 nm. PerCP is was historically used in flow cytometry, however it is highly susceptible to photobleaching and has poor stability. Alternatives like RealBlue 705 (BD Biosciences), NovaFluor Blue 690 or PerCP-eFluor™ 710 are preferred.
- What laser excites RayBright PerCP?
- RayBright PerCP is optimally excited by the 488 laser line. Its excitation maximum is 482 nm.
- How bright is RayBright PerCP?
- RayBright PerCP has a relative brightness of 1 out of 5.
- How many antibodies are available conjugated to RayBright PerCP?
- FluoroFinder tracks 34 commercially available RayBright PerCP conjugates, searchable by target, clone, host and application.